N6-甲基腺苷 T6599
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
25 mg | 1867-73-8 | ¥298.00 | 询底价 |
1 mL | 1867-73-8 | ¥460.00 | 询底价 |
500 mg | 1867-73-8 | ¥1,545.00 | 询底价 |
100 mg | 1867-73-8 | ¥671.00 | 询底价 |
50 mg | 1867-73-8 | ¥417.00 | 询底价 |
Product Introduction
Bioactivity
英文名: N6-methyladenosine
描述: N6-methyladenosine (NSC-29409) 是甲基化的腺嘌呤残基,苷是转移核糖核酸降解的内源性尿核苷产物。它是存在于所有高等真核生物中普遍的的信使 RNA 内部修饰,可修饰病毒 RNA,有抗病毒活性。
激酶实验: NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.
体外活性: N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA(snRNA) as well as several long non-coding RNA, such as Xist. [1] [2] [3] N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA). [4] N6-Methyladenosine is a widespread RNA modification in many tissues with high levels in the brain. N6-Methyladenosine is enriched near stop codons and within 3'UTRs in both mouse and human mRNAs. [5] The recent discovery that FTO, an obesity risk gene, encodes an m6A demethylase implicates m6A as an important regulator of physiological processes. [6]
体内活性: LD50: Mice >1 g/kg (i.g.). [7]
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: Ethanol : 2 mg/mL (7.11 mM)
DMSO : 56 mg/mL (199.09 mM)
H2O : 14 mg/mL (49.77 mM)
关键字: N-6-methyladenosine | inhibit | N6-Methyladenosine | Inhibitor | Endogenous Metabolite | N6methyladenosine | N6 methyladenosine | N6-methyladenosine | NSC 29409 | NSC29409 | Influenza Virus
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相关库: Anti-Tumor Natural Product Library | Nucleotide Compound Library | Human Endogenous Metabolite Library | Human Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Natural Product Library for HTS | RO5 Drug-like Natural Product Library | Gut Microbial Metabolite Library | Anti-infective Natural Product Library | Natural Product Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途