化合物 XI-006 T6610

Product Introduction
Bioactivity


英文名: XI-006

描述: XI-006 (XI-006) 是一种DNA 破坏剂、抗肿瘤剂和p53的激活剂。

细胞实验: MCF-7 cells treated with dimethyl sulfoxide (DMSO), nutlin-3a, or NSC-207895 are permeabilized with cold 70% ethanol overnight, and stained with a solution containing 50 μg/mL propidium iodide and 20 μg/mL RNase A at 37°C for 20 minutes. The cells are then subjected to flow cytometry analysis. The FlowJo software is used to calculate percentages of cells in each cell cycle phase. For terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) staining, MCF-7 cells treated with the NSC-207895 for 2 days are fixed with 4% paraformadelhyde for 1 hour, and then subjected to dUTP labeling using In Situ Cell Death Detection Kit TMR Red according to the manufacturer's protocol. For quantitation, at least 300 cells are randomly chosen and the numbers of TUNEL-positive cells are counted. (Only for Reference)

体外活性: XI-006 decreases both the MDMX mRNA and protein in MCF-7 cells. XI-006 induces expression of p53 as well as well-characterized p53-target gene, p21 and MDM2, in a dose-dependent manner in MCF-7 cells. XI-006 extends the half-life of p53 from 20 to 30 minutes to more than 3 hours as revealed by cycloheximide chase assays in MCF-7 cells. XI-006 also activates p53 and induces p21 and MDM2 expression in LNCaP prostate and A549 lung cancer cells. XI-006 increases the mRNA levels of proapoptotic genes including PUMA, BAX, and PIG3 in a dose-dependent manner in MCF-7 cells. XI-006 results in a significant increase in the numbers of sub-G0/G1 cells as well as G2 arrest. XI-006 also results in more than 40% of cells dying via apoptosis and decreases cell viability in A549 and LNCaP cells. [1] XI-006 inhibits biosynthesis of nucleic acids and proteins in L1210 cells. [2] XI-006 interacts with DNA repair to activate the DNA damage repair pathway in three species (S. cerevisiae, S. pombe and H. sapiens). [3] XI-006 acts as cytotoxic agent in the G/R-luc astrocytoma cell line with GI50 of 117 nM. [4]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 55 mg/ml (196.96 mM)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)


关键字: LPS | p53 | MDMX | XI006 | Inhibitor | NSC-207895 | inhibit | MDM2 | XI 006 | MDM-2/p53 | NSC207895

相关产品: Alrizomadlin | RITA | Ganoderic acid X | Tenovin-1 | MA242 | Serdemetan | SJ-172550 | UC2288 | p53-Mdm2 inhibitor 4 | Idasanutlin

相关库: Transcription Factor-Targeted Compound Library | Pyroptosis Compound Library | Anti-Lung Cancer Compound Library | Bioactive Compounds Library Max | Anti-Colorectal Cancer Compound Library | Anti-Cancer Active Compound Library | Apoptosis Compound Library | Anti-Pancreatic Cancer Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途