化合物Acetylcholine iodide T6751
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mL | 2260-50-6 | ¥148.00 | 询底价 |
200 mg | 2260-50-6 | ¥663.00 | 询底价 |
100 mg | 2260-50-6 | ¥442.00 | 询底价 |
500 mg | 2260-50-6 | ¥1,193.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Acetylcholine iodide
描述: Acetylcholine iodide (Acetylcolina) 是一种在中枢和外周神经系统中常见的神经递质。
细胞实验: The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.(Only for Reference)
体外活性: Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2].
体内活性: Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 50 mg/mL (183.08 mM)
H2O : 33 mg/mL (120.83 mM)
关键字: ACh | Inhibitor | Acetylcholine Iodide | Acetylcholine iodide | Acetylcholine | inhibit
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途