化合物BIO-acetoxime T6787
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mg | 667463-85-6 | ¥278.00 | 询底价 |
25 mg | 667463-85-6 | ¥1,990.00 | 询底价 |
100 mg | 667463-85-6 | ¥4,890.00 | 询底价 |
1 mL | 667463-85-6 | ¥728.00 | 询底价 |
5 mg | 667463-85-6 | ¥662.00 | 询底价 |
2 mg | 667463-85-6 | ¥393.00 | 询底价 |
50 mg | 667463-85-6 | ¥3,150.00 | 询底价 |
10 mg | 667463-85-6 | ¥1,070.00 | 询底价 |
Product Introduction
Bioactivity
英文名: BIO-acetoxime
描述: BIO-acetoxime (GSK-3 Inhibitor X) 是一种 GSK3α/β 抑制剂,IC50值均为 10 nM,具有抗惊厥和抗感染作用。
细胞实验: Cell viability is analyzed by CellTiter 96 AQueous One Solution Cell Proliferation Assay according to manufacturer's instructions, approximately 5,000 cells are plated per well of 96-well tissue culture plates with 100 μL of medium. To assess cell viability, rather than proliferation rate, inhibitor- and control-treated cells are assayed after the same growth time had elapsed. The results represent the mean ± SD of triplicate samples, expressed as a percentage of control.(Only for Reference)
激酶实验: Kinase Assays: Kinases activities are assayed in Buffer A or C, at 30 °C, at a final ATP concentration of 15 μM. Blank values are subtracted and activities calculated as pmoles of phosphate incorporated for a 10 min incubation. The activities are expressed in % of the maximal activity, i.e., in the absence of inhibitors. Controls are performed with appropriate dilutions of DMSO. GSK-3α/β is purified from porcine brain by affinity chromatography on immobilized axin. It is assayed, following a 1/100 dilution in 1 mg BSA/mL 10 mM DTT, with 5 μL 40 μM GS-1 peptide as a substrate, in buffer A, in the presence of 15 μM [γ-33P] ATP (3000 Ci/mmol; 1 mCi/mL) in a final volume of 30 μL. After 30 min incubation at 30 °C, 25 μL aliquots of supernatant are spotted onto 2.5 × 3 cm pieces of Whatman P81 phosphocellulose paper, and, 20 s later, the filters are washed five times (for at least 5 min each time) in a solution of 10 mL phosphoric acid/liter of water. The wet filters are counted in the presence of 1 mL of ACS scintillation fluid. CDK1/cyclin B is extracted in homogenization buffer from M phase starfish (Marthasterias glacialis) oocytes and purified by affinity chromatography on p9CKShs1-sepharose beads, from which it is eluted by free p9CKShs1. The kinase activity is assayed in buffer C, with 1 mg histone H1 /mL, in the presence of 15 μM [γ-32P] ATP (3000 Ci/mmol; 1 mCi/mL) in a final volume of 30 μL. After 10 min incubation at 30 °C, 25 μL aliquots of supernatant are spotted onto P81 phosphocellulose papers and treated as described above. CDK5/p25 is reconstituted by mixing equal amounts of recombinant mammalian CDK5 and p25 expressed in E. coli as GST (Glutathione-S-transferase) fusion proteins and purified by affinity chromatography on glutathione-agarose (p25 is a truncated version of p35, the 35 kDa CDK5 activator). Its activity is assayed in buffer C as described for CDK1/cyclin B.0
体外活性: In human oral epithelial cells, BIO-acetoxime suppresses viral gene expression and protects oral epithelial cells from HSV-1 infection. [2] In SY5Y-MYCN cells, BIO-acetoxime strongly reduces c-MYC expression and p-SMAD3 levels. BIO-acetoxime also decreases cell viability of KCN, KCNR, SY5Y, Kelly, and IMR32 cells by mediating apoptosis. [3] In HEK 293T cells, BIO-acetoxime is also found to reduce antiviral innate immunity downstream of IRF3 activation by inhibition of GSK3α/β activities. [4]
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 8 mg/mL (20 mM)
关键字: BIO acetoxime | Glycogen synthase kinase 3 | inhibit | Herpes simplex virus | Glycogen synthase kinase-3 | BIO-acetoxime | HSV | GSK-3 | Apoptosis | Inhibitor | BIOacetoxime
相关产品: TCS JNK 5a | (S)-(+)-Rolipram | DEL-22379 | PU02 | EGFR-IN-12 | Glycochenodeoxycholic Acid | Hexylresorcinol | TZ9 | Diatrizoic Acid | RSH-7
相关库: Anti-Neurodegenerative Disease Compound Library | Anti-Cancer Metabolism Compound Library | Anti-Cancer Compound Library | Neuronal Differentiation Compound Library | Inhibitor Library | Kinase Inhibitor Library | Highly Selective Inhibitor Library | Reprogramming Compound Library | Stem Cell Differentiation Compound Library | Anti-Infection Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途