5-溴脱氧尿嘧啶核苷 T6794

Product Introduction
Bioactivity


英文名: 5-BrdU

描述: 5-BrdU (Broxuridine) 是一种核苷类似物，用于检测增殖细胞并与胸苷竞争掺入 DNA。

细胞实验: Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 μM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.(Only for Reference)

激酶实验: Assays are run in the presence of 100 μM ATP using 10 μM of substrate. 30 μL PROTEIN-MIX in 25% DMSO and incubated for 15 min at room temperature. 10 μL PEPTIDE-MIX is added, the mixture is incubated for 60 min at RT and stopped by adding 180 μL 6.4% TCA (final concentration: 5%). Incorporated phosphate is measured in a scintillation counter and IC50 values are calculated using a sigmoidal curve analysis program with variable hill slope[1].

体外活性: In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. [1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. [2]

体内活性: In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. [1]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: Ethanol : 2 mg/mL(6.5 mM)
H2O : 15.4 mg/mL(50 mM)
DMSO : 50 mg/mL (162.81 mM)


关键字: 5BrdU | inhibit | Nucleoside Antimetabolite/Analog | 5-BrdU | Inhibitor | 5 BrdU

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途