腔肠素 T6808

Product Introduction
Bioactivity


英文名: Coelenterazine

描述: Coelenterazine (Coelenteramine) 是一种 apoaequorin 和 Renilla 荧光素酶的发光酶底物。它是超氧阴离子敏感化学发光探针，可用于检测过氧亚硝酸盐的化学发光。其中 Renilla 荧光素酶和底物 coelenterazine 被用作生物发光共振能量转移中检测蛋白质-蛋白质相互作用的生物发光供体。

细胞实验: Cells are plated at a density of 5×10^4?cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2?and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only.

动物实验: Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only.

体外活性: When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2]

体内活性: 在4T1植入的小鼠中，Coelenterazine检测与癌症相关的超氧阴离子，并用于评估超氧阴离子在癌细胞生物学及其病理作用中的角色。[2]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 78 mg/mL (184.2 mM)
Ethanol : 1 mg/mL (2.36 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)


关键字: inhibit | Inhibitor | Coelenterazine

相关库: Marine Natural Product Library | Polyphenolic Natural Product Library | Bioactive Compounds Library Max | Human Metabolite Library | RO5 Drug-like Natural Product Library | Preclinical Compound Library | Anti-Cardiovascular Disease Compound Library | NO PAINS Compound Library | Natural Product Library | Bioactive Compound Library

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