亚甲蓝 T6888
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
50 mg | 61-73-4 | ¥239.00 | 询底价 |
500 mg | 61-73-4 | ¥713.00 | 询底价 |
100 mg | 61-73-4 | ¥418.00 | 询底价 |
1 mL | 61-73-4 | ¥164.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Methylene Blue
描述: Methylene Blue (Tetramethylthionine chloride) 是鸟苷酸环化酶,是单胺氧化酶 A 和 NO 合酶抑制剂。它有抗伤害感受、抗疟疾、抗抑郁和抗焦虑作用,可研究高铁血红蛋白血症、神经退行性疾病和异环磷酰胺引起的脑病。它是血管加压剂,用作色素内窥镜检查中的染料。
细胞实验: Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm.
激酶实验: Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically.
动物实验: MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s.
体外活性: MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores.
体内活性: Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery.
存储条件: keep away from direct sunlightPowder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: H2O : 3.2 mg/mL (10 mM)
DMSO : 1mg/ml
关键字: antimalarial | anxiolytic | inhibit | Parasite | Methylthioninium | methemoglobinemias | Monoamine Oxidase | cGMP | CI 52015 | Nitric oxide synthases | NOS | Tetramethylthionine Chloride | Inhibitor | antinociception | CI52015 | antidepressant | Methylene Blue | dye | methylthioninium Chloride | MAO | vasopressor | Basic Blue-9 | Guanylate Cyclase | Basic Blue9 | NO Synthase
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相关库: Drug Repurposing Compound Library | Anti-Neurodegenerative Disease Compound Library | Microtubule-Targeted Compound Library | Anti-Cancer Drug Library | Membrane Protein-targeted Compound Library | Inhibitor Library | GPCR Compound Library | Anti-Cancer Clinical Compound Library | Anti-Cancer Approved Drug Library | Anti-Parasitic Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途