化合物NSC 95397 T7078

Product Introduction
Bioactivity


英文名: NSC 95397

描述: NSC 95397 是一种选择性的Cdc25双特异性磷酸酶抑制剂。它抑制丝裂原活化蛋白激酶磷酸酶-1(MKP-1)，通过 MKP-1 和 ERK1/2 途径抑制结肠癌细胞的增殖并诱导细胞凋亡。

细胞实验: To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates.?After overnight culture, NSC 95397 cells were added at the indicated concentrations.?After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit.?O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD.?Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1]

体外活性: NSC 95397 通过调节细胞周期相关蛋白，包括p21、cyclin-dependent kinases和caspases[1]，抑制增殖并诱导凋亡，从而降低了三种结肠癌细胞系的细胞活性和非依赖性生长。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 100 mg/mL (322.18 mM)


关键字: Apoptosis | NSC-95397 | Inhibitor | inhibit | Phosphatase | NSC 95397 | NSC95397

相关产品: Clitocine | Trigonelline | SW106065 | Acacetin | Ginsenoside Rh2 | LDCA | [8]-Shogaol | MK-2206 dihydrochloride | Anticancer agent 133 | PRIMA-1

相关库: Anti-Cancer Compound Library | Bioactive Compounds Library Max | Phosphatase Inhibitor Library | Kinase Inhibitor Library | Inhibitor Library | Anti-Colorectal Cancer Compound Library | Anti-Cancer Active Compound Library | Apoptosis Compound Library | Metabolism Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途