2-亚氨基生物素盐酸盐 T7206L
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
10 mg | 76985-52-9 | ¥1,230.00 | 询底价 |
2 mg | 76985-52-9 | ¥469.00 | 询底价 |
1 mL | 76985-52-9 | ¥961.00 | 询底价 |
100 mg | 76985-52-9 | ¥5,280.00 | 询底价 |
50 mg | 76985-52-9 | ¥3,660.00 | 询底价 |
5 mg | 76985-52-9 | ¥776.00 | 询底价 |
1 mg | 76985-52-9 | ¥327.00 | 询底价 |
25 mg | 76985-52-9 | ¥2,220.00 | 询底价 |
Product Introduction
Bioactivity
英文名: 2-Iminobiotin hydrobromide
描述: 2-Iminobiotin hydrobromide (Guanidinobiotin) 是生物素 (维生素 H 或 B7) 类似物。它是一种可逆的 NO 合酶抑制剂,可作用于小鼠 iNOS(Ki:21.8 μM)和大鼠 n-cNOS(Ki:37.5 μM)。它和低温环境均可以保护人类神经细胞,使其免受缺氧诱导的细胞损伤。
细胞实验: In vitro hypoxia was induced for 7 h in IMR-32 cell cultures by using our recently described system with minor modifications.?Enzyme stock solutions (100x) of catalase and glucose oxidase ?were diluted in cell culture medium (DMEM/F12, 1% FCS;?final concentration: 120 and 2 U/ml respectively).?A rapid decrease of partial pressure of oxygen (pO2) to levels below 10 mmHg was achieved by adding the enzymes to glucose containing culture medium.?Also a decline in glucose (<1 g/l) and pH (<7.0) was observed, resembling the clinical characteristics of hypoxic-ischemic injury in vivo.?Hypoxic conditions were confirmed with a tissue oxygen pressure monitor .?After the hypoxic insult, cells were washed twice with PBS and cultures were placed into an incubator at 33.5°C (hypothermia) employing culture medium with (i) solvent (citrate buffer 1%) or (ii) 2-IB at 10, 30, 50, 100, and 300 ng/ml.?To determine the optimal “reperfusion” time, a time-interval curve investigating cell damage (LDH release) was performed.?Analyses of LDH release, ROS generation, hydrogen peroxide release, metabolic activity, cell signaling, apoptosis-related protein expression/activity and expression analysis of 35 human apoptosis-related proteins were performed at different time points post-hypoxia[1]
体外活性: All cultures were subjected to 25 h of hypothermia (33.5°C), and incubated with vehicle or 2-iminobiotin (2-IB) (10, 30, 50, 100, and 300 ng/ml).?Cell morphology was evaluated by brightfield microscopy.?Cell damage was analyzed by LDH assays.?Production of reactive oxygen species (ROS) was measured using fluorometric assays.?Western blotting for PARP, Caspase-3, and the phosphorylated forms of akt and erk1/2 was conducted.?To evaluate early apoptotic events and signaling, cell protein was isolated 4 h post-hypoxia and human apoptosis proteome profiler arrays were performed.?Twenty-five hour after the hypoxic insult, clear morphological signs of cell damage were visible and significant LDH release as well as ROS production were observed even under hypothermic conditions.?Post-hypoxic application of 2-IB (10 and 30 ng/ml) reduced the hypoxia-induced LDH release but not ROS production.?Phosphorylation of erk1/2 was significantly increased after hypoxia, while phosphorylation of akt, protein expression of Caspase-3 and cleavage of PARP were only slightly increased.?Addition of 2-IB did not affect any of the investigated proteins.?Apoptosis proteome profiler arrays performed with cellular protein obtained 4 h after hypoxia revealed that post-hypoxic application of 2-IB resulted in a ≥ 25% down regulation of 10/35 apoptosis-related proteins: Bad, Bax, Bcl-2, cleaved Caspase-3, TRAILR1, TRAILR2, PON2, p21, p27, and phospho Rad17[1].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 12.5 mg/mL (51.37 mM)
关键字: Nitric oxide synthases | NOS | 2-Iminobiotin Hydrobromide | NO Synthase | 2-Iminobiotin | 2Iminobiotin hydrobromide | 2 Iminobiotin hydrobromide | Inhibitor | inhibit
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相关库: Drug Repurposing Compound Library | ReFRAME Related Library | Clinical Compound Library | Bioactive Compounds Library Max | Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library
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