化合物RN-1734 T7572
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
500 mg | 946387-07-1 | ¥6,250.00 | 询底价 |
50 mg | 946387-07-1 | ¥1,930.00 | 询底价 |
25 mg | 946387-07-1 | ¥1,280.00 | 询底价 |
100 mg | 946387-07-1 | ¥2,880.00 | 询底价 |
10 mg | 946387-07-1 | ¥638.00 | 询底价 |
1 mL | 946387-07-1 | ¥543.00 | 询底价 |
2 mg | 946387-07-1 | ¥253.00 | 询底价 |
5 mg | 946387-07-1 | ¥455.00 | 询底价 |
Product Introduction
Bioactivity
英文名: RN-1734
描述: RN-1734是TRPV4通道的选择性拮抗剂,拮抗 4αPDD 介导的 TRPV4 活化,作用于 hTRPV4, mTRPV4, 和 rTRPV4的IC50分别为 2.3 μM、5.9 μM、3.2 μM。RN-1734能显著降低白细胞介素 1β和肿瘤坏死因子 α 的产生,而不改变 olig2- 阳性细胞的数量。
细胞实验: LPS-treated microglial cells were further divided into a LPS group, a vehicle (DMSO treatment) group and a RN-1734 group.?The cells were treated with 1 μg/ml LPS (Sigma) or RN-1734 (10 μM) for 3 h .?Next, the cells were triple-washed with DMEM medium to remove LPS, and then incubated in DMEM with or without RN1734 for 24 h. Finally, the cells were collected for TRPV4 immunocytostaining, and the supernatant was collected separately for enzyme-linked immunosorbent assay (ELISA) or for the oligodendrocyte apoptosis experiment.?The supernatant of the microglial cells was mixed with DMEM/F12 at a ratio of 1:1.?The mixed medium without LPS was used for the control (Ctrl) group, the medium with LPS only was used for the conditional medium (CM) group, and the media with LPS and DMSO or RN-1734 were used for the CM-vehicle group and the CM-RN1734 group, respectively.?After oligodendrocytes grew for 2 days in 6-well plates in DMEM/F12 containing 10% FBS, the differentiation medium was replaced with the described Ctrl or different CM media for 24 h. Then, the cells were harvested for apoptosis analysis and western blot analysis separately[1].
体外活性: In vitro, RN-1734 treatment clearly inhibited the influx of calcium and decreased the levels of IL-1β and TNF-α in lipopolysaccharide (LPS)-activated microglial cells by suppressing NF-κB P65 phosphorylation.?Apoptosis of oligodendrocyte induced by LPS-activated microglia was also alleviated by RN-1734.?Activation of TRPV4 in microglia is involved in oligodendrocyte apoptosis through the activation of the NF-κB signaling pathway, thus revealing a new mechanism of CNS demyelination[1].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 50 mg/mL (141.52 mM)
关键字: Inhibitor | Transient receptor potential channels | TRP Channel | RN-1734 | RN 1734 | RN1734 | inhibit
相关产品: Linoleoyl Ethanolamide | TRPV4 agonist-1 | PF-05105679 | (+)-Camphor | AMG2850 | FEMA-4809 | PF-04745637 | MK-2295 | Mesendogen | Methyl Kakuol
相关库: Ion Channel Inhibitor Library | ReFRAME Related Library | Anti-Cancer Compound Library | Membrane Protein-targeted Compound Library | Bioactive Compounds Library Max | Neuronal Signaling Compound Library | Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途