阿魏酸甲酯 TL0004

Product Introduction
Bioactivity


英文名: FERULIC ACID METHYL ESTER

描述: FERULIC ACID METHYL ESTER (Methyl ferulate) 是从大百部中分到的阿魏酸衍生物，具有抗氧化和抗炎作用。在原发性骨髓源性巨噬细胞中，它抑制 COX-2 的表达，降低 p-p38和 p-JNK 的水平。它能够透过细胞膜和血脑屏障，清除自由基，可研究神经退行性疾病。

细胞实验: Hydrogen peroxide (8.8 M solution) stored at 4 C was firstdiluted in PBS to prepare a 100 mM solution on the day of theexperiment. This was further diluted in growth medium to preparethe final working solution. PC12 cells were plated in collagencoated96-well microplates at a density of 5 105 cells/ml(100 μl per well). Blank wells contained only growth medium forbackground correction. After 48 h of incubation to allow for cellattachment, 20 μl of growth medium supplemented with differentconcentrations of HCAs were added in triplicate wells and preincubatedfor 1 h. Maximum concentration of DMSO in the wellswas kept below 0.2%. Afterwards, 20 μl of H2O2 solution was added.The concentration of H2O2 in the well was 75 μM. After anotherhour, the medium was replaced with fresh one and cells wereincubated overnight. In the end, the medium was replaced with30 μl of MTT 0.5 mg/ml dissolved in RPMI without phenol red.Formazan crystals were solubilised in 200 μl DMSO after 1.5 h ofincubation at 37 C.

激酶实验: DPPH radical scavenging activity of caffeic acid and ferulic acid derivatives was assessed as previously described withsome modifications. Briefly, the test compound was dissolved in dimethyl sulfoxide and 4 different concentrations weremixed with a methanolic solution of DPPH 100 mM in duplicate. After 30 min of incubation at room temperature in the dark, theabsorbance at 517 nm was measured by a spectrophotometer . The concentrations (in the range 1-100 μM) were carefully chosen for each compound in order to produce a suitable doseeresponse curve. The percent inhibition of the radical was calculated based on the absorbance of the mixture compared to the absorbance of DPPH solution alone.

体外活性: Ferulic acid methyl ester has less antioxidant capacity than ferulic acid in neuronal PC12 cells (IC50 = 74.7 μM for ferulic acid ethyl ester vs. 44.6 μM for ferulic acid, 2,2-diphenyl-1-picrylhydrazyl radical scavenging). Ferulic acid methyl ester, at 10-25 μg/ml, inhibits the release of pro-inflammatory cytokines, blocks the expression of COX-2, and reduces nitric oxide generation from LPS-stimulated macrophages.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (240.14 mM)
Ethanol : 20 mg/mL


关键字: Inhibitor | inhibit | FERULIC ACID METHYL ESTER | p38 MAPK | Autophagy

相关产品: SB 202190 | PH-797804 | Lucidenic acid D | AMG-47a | Chromium picolinate | Adezmapimod | Neflamapimod | PD 169316 | Rotundic acid | AMG-548 dihydrochloride (864249-60-5 free base)

相关库: PBCRBS Traditional Chinese Medicine Compound Library | Tobacco Monomer Library | Chinese Pharmacopoeia Natural Product Library | Bioactive Compounds Library Max | Neural Regeneration Compound Library | Anti-Ovarian Cancer Compound Library | Kinase Inhibitor Library | Cytoskeletal Signaling Pathway Compound Library | Traditional Chinese Medicine Monomer Library | Traditional Mongolian Medicine Compound Library

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