佛波醇12-十四酸酯13-乙酸酯 TQ0198
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
50 mg | 16561-29-8 | ¥5,530.00 | 询底价 |
1 mL | 16561-29-8 | ¥1,680.00 | 询底价 |
25 mg | 16561-29-8 | ¥3,860.00 | 询底价 |
5 mg | 16561-29-8 | ¥1,050.00 | 询底价 |
100 mg | 16561-29-8 | ¥7,720.00 | 询底价 |
1 mg | 16561-29-8 | ¥436.00 | 询底价 |
10 mg | 16561-29-8 | ¥1,770.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Phorbol 12-myristate 13-acetate
描述: Phorbol 12-myristate 13-acetate (PMA) 属于佛波酯类天然产物,是一种 PKC、SphK、NF-κB 的激活剂。Phorbol 12-myristate 13-acetate 可诱导 THP1 细胞分化。
细胞实验: αT3-1 and LβT-2 cells are grown in monolayer cultured in DMEM in humidified incubator 5% CO2 at 37°C. Serum starvation is with 0.1% FCS in the same medium for 16 h. GnRH and PMA are then added for the length of time as indicated. In general, αT3-1 cells are transiently transfected by ExGen 500 or by jetPRIME, while LβT2 cells only by jetPRIME transfection reagent. For experiments with dominant-negative (DN) PKCs, αT3-1 cells (in 6 cm plates) are transfected with 1.5 μg of p38α-GFP with 3 μg of control vector, pCDNA3, or with 3 μg of the DN-PKCs constructs. For LβT2 cells, transfections are performed (in 10 cm plates) with 4 μg of p38α-GFP along with 9 μg of control vector, pCDNA3, or with 9 μg of the DN-PKCs constructs. Approximately 30 h after transfection, the cells are serum-starved (0.1% FCS) for 16 h and later stimulated with GnRH or PMA, washed twice with ice-cold PBS, treated with the lysis buffer, followed by one freeze-thaw cycle. Cells are harvested; following centrifugation (15,000×g, 15 min, 4°C) supernatants are taken for immunoprecipitation experiments [2].
动物实验: All experiments are performed with male Wistar rats (weighing 250-280 g). One hundred and thirty-five Wistar rats are randomly divided into seven groups. (1) Rats in the sham group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline; (2) Rats in the IR group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline 30 min before middle cerebral artery occlusion (MCAO); (3) Rats in the Carbenoxolone (CBX) group (n=21) are given a lateral cerebral ventricle injection of CBX (5 μg/mL×10 μL) 30 min before MCAO; (4) Rats in the Sch-6783 group (n=21) are given a lateral cerebral ventricle injection of DZX (2 mM×30 μL) 30 min prior to MCAO; (5) Rats in the 5-HD group (n=21) are given a lateral cerebral ventricle injection of 5-HD (100 mM×10 μL), and after 10 min, DZX is injected 15 min prior to MCAO; (6) The rats in the DZX + Ro group (n=15) are given a lateral cerebral ventricle injection of DZX, and after 10 min, Ro-31-8425 (400 μg/kg) is injected 15 min prior to MCAO; (7) The rats in the 5-HD+PMA group (n=15) are given an intraperitoneal injection of PMA (200 μg/kg) after the injection of 5-HD and DZX [3].
体外活性: 方法:球体培养的人类黑色素瘤细胞 WM 系列用 Phorbol 12-myristate 13-acetate (50 ng/mL) 处理 3 天,使用 MTS 方法检测细胞生长情况。结果:Phorbol 12-myristate 13-acetate 促进黑色素瘤细胞增殖,WM35 细胞的细胞数提高到 265%。[1]方法:人单核白血球细胞 THP-1 用 Phorbol 12-myristate 13-acetate (200 ng/mL) 处理 1-5 天,使用光学显微镜评估形态,使用 Flow Cytometry 方法检测靶点表达。结果:Phorbol 12-myristate 13-acetate 诱导 THP-1 细胞分化为巨噬细胞样细胞 (THP-1 巨噬细胞),CD11 和 CD14 的细胞表面表达增加。[2]方法:人静脉内皮细胞 HUVECs 用 Phorbol 12-myristate 13-acetate (10-40 ng/mL) 处理 8 h,使用 Wound healing migration assay 检测细胞迁移情况。结果:短期 Phorbol 12-myristate 13-acetate 处理可增强内皮细胞迁移。[3]
体内活性: 方法:为研究佛波酯对啮齿类动物大脑发育的影响,将 Phorbol 12-myristate 13-acetate (100-500 μg/kg) 单次腹腔注射给药给缺乏 IL-18 或 IRAK-4 的新生大鼠和小鼠,24 h、7 天或14 天后处死动物。结果:Phorbol 12-myristate 13-acetate 在大脑中诱导炎症反应并引起广泛的神经退行性变。缺乏 IL-18 或 IRAK-4 对 Phorbol 12-myristate 13-acetate 诱导的脑损伤有保护作用。[4]方法:为构建急性小鼠耳炎症模型,将 Phorbol 12-myristate 13-acetate (20 μL 125 μg/mL PMA 丙酮溶液) 局部处理 CD-1 小鼠双耳,风干并完全吸收。结果:用 Phorbol 12-myristate 13-acetate 攻击的耳组织在施用约 2 小时开始出现炎症迹象,包括肿胀和发红。[5]
存储条件: store at low temperature,keep away from direct sunlightPowder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 60 mg/mL (97.27 mM)
H2O : Insoluble
5% DMSO+95% Saline : 2.5 mg/mL (4.05 mM, suspension)
关键字: PKC | SphK | Nuclear factor-κB | Protein kinase C | Inhibitor | Phorbol 12 myristate 13 acetate | TPA | inhibit | Phorbol 12myristate 13acetate | Phorbol myristate | Nuclear factor-kappaB | Sphingosine kinase | Phorbol 12-myristate | NF-κB | Phorbol 12-myristate 13-acetate
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