萝卜硫素 TQ0207

Product Introduction
Bioactivity


英文名: Sulforaphane

描述: Sulforaphane 是存在于多种蔬菜中的一种天然异硫氰酸酯。它可以激活Nrf2，并通过 AMPK 依赖性信号传导抑制高糖诱导的胰腺癌。它增加肿瘤抑制蛋白的转录并抑制组蛋白脱乙酰酶的活性，具有抗癌和抗炎活性。

细胞实验: HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1].

动物实验: At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3].

体外活性: Sulforaphane以剂量依赖的方式引起细胞周期阻滞，随后导致细胞死亡。这种由sulforaphane引起的细胞周期阻滞与cyclins A和B1表达增加有关。Sulforaphane通过凋亡过程诱导细胞死亡，并抑制HT29静息结肠癌细胞的生长重启及降低细胞活性，对分化的CaCo2细胞毒性较低[1]。H9c2大鼠心肌细胞预处理sulforaphane可减少凋亡细胞数和凋亡蛋白表达，并降低多柔比星引起的线粒体膜电位增加。此外，sulforaphane增加血红素加氧酶-1的mRNA和蛋白表达，从而降低多柔比星引起的线粒体反应性氧种类水平[2]。

体内活性: 给予sulforaphane能够降低单剂量DMBA诱发的雌性SD大鼠乳腺肿瘤的发生率、数量、体重，并延缓其发展[3]。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 55 mg/mL (310.23 mM)


关键字: Keap1-Nrf2 | inhibit | Inhibitor | HDAC | Histone deacetylases | Apoptosis | Sulforaphane

相关产品: BG45 | GSK1904529A | LCH-7749944 | COTI-2 | Omaveloxolone | ML141 | Voreloxin hydrochloride | Coenzyme Q9 | Larotrectinib sulfate | Nocodazole

相关库: Drug Repurposing Compound Library | Anti-Cancer Compound Library | Anti-Cancer Drug Library | Ferroptosis Compound Library | Anti-Cancer Clinical Compound Library | Epigenetics Compound Library | Anti-Cancer Active Compound Library | DNA Damage & Repair Compound Library | Natural Product Library | Chromatin Modification Compound Library

萝卜硫素 TQ0207信息由TargetMol中国为您提供，如您想了解更多关于萝卜硫素 TQ0207报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途