卡泊三醇一水合物 TQ0244
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
25 mg | 147657-22-5 | ¥1,550.00 | 询底价 |
1 mg | 147657-22-5 | ¥233.00 | 询底价 |
10 mg | 147657-22-5 | ¥778.00 | 询底价 |
50 mg | 147657-22-5 | ¥2,890.00 | 询底价 |
100 mg | 147657-22-5 | ¥4,370.00 | 询底价 |
5 mg | 147657-22-5 | ¥523.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Calcipotriol monohydrate
描述: Calcipotriol monohydrate 是维生素 D3 的类似物,对维生素 D 受体具有高亲和力。Calcipotriol monohydrate 可用于银屑病研究。
细胞实验: Normal human epidermal keratinocytes are grown in serum-free keratinocyte growth medium Epilife and used at the third passage in all experiments. The growth supplement is omitted 48 h before experiments. As a control, IL-17A and IL-22 are either added or not added to the cells. Cultured NHEK cells are stimulated with IL-17A (200 ng/mL) and/or IL-22 (200 ng/mL) followed by co-incubation in the presence or absence of Calcipotriol at 0.2-40 nM to test its modulatory effect. Cells are harvested 3 days later and subjected to real-time qPCR. Culture supernatants are also collected and frozen at -80°C until used for ELISA [1].
动物实验: The 160 female SKH-1 hairless mice (6-7 weeks of age) are used. After UV treatment, mice without tumors are randomly divided into five groups, four chemoprevention groups (Diclofenac plus DFMO; Diclofenac plus Calcipotriol; DFMO plus calcitriol; and Diclofenac plus DFMO plus Calcipotriol) and one placebo group (skin lotion). The mice are treated with test mixtures once a day, five days a week, for a total of 17 weeks. The test mixtures are applied topically on the dorsal surface of the mice. Ten microliters are applied by a pipette after which the mixture is rubbed onto the skin. This corresponded to the following doses of each active substance in the treatments: 100 μg/week for Diclofenac (30 mg/g undiluted), 0.166 μg/week for Calcitriol (50 μg/g undiluted), and 463.3 μg/week for DFMO (139 mg/g undiluted) [3].
体外活性: Calcipotriol monohydrate(100, 10, 和 1 ng/mL)显著提升了NK细胞表面NKp30的表达量,在4小时的孵育后[1]。当NHEK细胞未被IL-17A或IL-22刺激时,Calcipotriol monohydrate轻微增强(0.2 nM)IL-8 mRNA表达,或无影响(2-20 nM)。加入IL-17A和IL-22后,IL-8的mRNA表达显著提升,证实了我们之前的研究。这种IL-8 mRNA表达的增强,通过Calcipotriol monohydrate(2, 20 和 40 nM)以剂量依赖方式被抑制[2]。
体内活性: 在经过Diclofenac加Calcipotriol monohydrate(p=0.018)和Diclofenac加DFMO加Calcipotriol monohydrate(p=0.002)处理的组别中,与安慰剂(线性回归模型)[3]相比,体重增加显著较小。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: Ethanol : 95 mg/mL (220.61 mM)
DMSO : 95 mg/mL (220.61 mM)
关键字: VD/VDR | Vitamin D receptor | Calcipotriol | Vitamin D | Calcipotriol monohydrate | Calcipotriol Monohydrate | inhibit | Inhibitor
相关库: Drug Repurposing Compound Library | Approved Drug Library | Bioactive Compounds Library Max | EMA Approved Drug Library | Metabolism Compound Library | Bioactive Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途