内吗啡肽 1 TQ0267

Product Introduction
Bioactivity


英文名: Endomorphin 1

描述: Endomorphin 1 是一种高亲和力和选择性的 μ-阿片受体激动剂，对 kappa3 结合位点具有合理的亲和力 ，Ki 为 20 到 30 nM 之间。

激酶实验: 125I-Endomorphin 1 or 125I-Endomorphin 2 binding (0.2 nM) is performed in potassium phosphate buffer (50 mM, pH 7.4; 0.5 mL) with MgCl2 (5 mM) at a tissue concentration of 10 mg wet weight/mL for brains or 0.06 mg protein/mL for MOR-1/CHO cells. Specific binding is determined in the presence and absence of either 1 μM of the corresponding unlabeled peptide. The entire mixture is then incubated at 25°C for 1 hr and filtered over no. 32 glass fiber filters which have been presoaked for 1 hr in 0.5% polyethyleneimine and washed twice with ice-cold Tris buffer using a Brandel cell harvester. The filters are then counted on a Packard Cobra gamma counter. The other opioid receptor binding assays are performed.

动物实验: Groups of mice are treated i.c.v. with Endomorphin 1 (12 μg) or Endomorphin 2 (3 μg) 15 min before a 0.5-cc charcoal meal (2.5% gum tragacanth,10% activated charcoal in water). The mice are killed 30 min later and the distance the charcoal traveled is measured.

体外活性: Endomorphin是一种内源性阿片肽，也是两种Endomorphins之一。它是μ-阿片受体的高亲和力、高选择性激动剂，并且与Endomorphin 2一同作用。Endomorphin 1和Endomorphin 2都能强效地竞争μ1与μ2受体位点。Endomorphins对delta或kappa1结合位点的亲和力很低，其Ki值均大于500 nM。

体内活性: Endomorphin 1和Endomorphin 2均为有效的镇痛剂，其效果分别在10分钟和15分钟达到顶峰。所有后续研究均在达到最大效应时进行。这两种化合物在脊髓上方和脊髓内均完全活跃，且没有出现效应上限。Endomorphin 1和Endomorphin 2的作用特征与吗啡相似。在对CD-1小鼠产生超过70%镇痛效果的剂量下，这两种化合物在CXBK小鼠中均无镇痛活性。

存储条件: keep away from moisturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 24 mg/mL (39.3 mM)


关键字: inhibit | Endomorphin1 | Opioid Receptor | Inhibitor | Endomorphin 1

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